The potential of siphonodon celastrineus griff, traditional medicinal plants of thailand for treatment from local wisdom knowledge lead to the development of sustainable use

Abstract

The research project on The potential of Siphonodon celastrineus Griff, traditional medicinal plants of Thailand for treatment from local wisdom knowledge lead to the development of sustainable use, with the objective of studying the botanical, phytochemical, and biological activity of the Siphonodon celastrineus to create confidence in the use of S. celastrineus in herbal medicine by using the bark, leaves and fruit of the S. celastrineus to extract with 3 types of solvents. Each of hexane, ethyl acetate, and methanol, respectively, from low to high polarity. All nine extracts will be extracted. Preliminary examinations for phytochemistry, Antioxidant activity by DPPH radical scavenging assay and quantitative determination of phenolic compounds by Folin-Ciocalteu method and antimicrobial activity were tested. The research found that. 1. The extract of the results of the methanol layer of the S.celastrineus shows a reduction in the amount of 1,1-diphenol-2-picrylhydrazyl (DPPH) had the highest EC50 equal to 31 mg/ml. The amount of phenol compounds found that the extracts from the methanol extract of S. celastrineus contain the amount of phenol. The highest concentration of 95.23 mg GAE / g dw 2. Extract from the bark of the S. celastrineus with methanol showed the best inhibition of the growth of Vibrio paraheamolyticus with the MIC value of 15.60 mg/ml. The extracts of the S. celastrineus leaf extracts with hexane showed the best inhibition of the growth of Vibrio cholerae with the MIC value equal to 31.25 mg/ml and leaf extracts with ethyl acetate showed the best inhibitory effect on the growth of Vibrio cholerae with a MIC value of 31.25 mg/ml and the extract of the fruit with ethyl acetate to inhibit the growth of Vibrio paraheamolyticus most with MIC equal to 15.60 mg/ml.