Isolation, classification and study of the influence of glow discharge plasma on dirty panicle rice fungal pathogens

Abstract

This study was conducted to isolate and classify fungal dirty panicle disease pathogens from five rice varieties. Observation of rice seeds under stereo microscope showed that the seeds from all five varieties had 100% lesions of dirty panicle disease. When isolating the fungal pathogen with three methods, i.e. (i) ground rice seeds shaken in dH2O and then spread on potato dextrose agar (PDA), (ii) rice seeds washed with dH2O twice, then placed on PDA, and (iii) rice seeds shaken in 5% clorox for 5 min, after that washed with dH2O twice and then placed on PDA, 57 isolates of fungi were obtained. Morphological characterization classified the fungi into 17 genera, i.e. Alternaria, Arthrinium, Aspergillus, Biporalis, Botryotinia, Botrytis, Corynespora, Curvularia, Cylindrocarpon, Fusarium, Humicola, Monilinia, Mucor, Penicillium, Rhizopus, Stachybotrys and Stemphylium. Four genera, i.e. Alternaria, Bipolaris, Curvularia and Fusarium were reported to cause of dirty panicle disease of rice. The effect of glow discharge plasma on inhibition of fungal pathogen showed that exposed glow discharge plasma for 150s could inhibit mycelium growth of Curvularia but did not affect to Bipolaris and Fusarium.